APTOMERLAR TEXNOLOGIYASINI BIOTEXNOLOGIYADA QO`LLASH
Keywords:
aptamer; SELEX; in vitro tanlovi; aptasensor; diagnostika; biosensor.Abstract
So'nggi paytlarda aptamerlar ko'plab olimlarning e'tiborini tortdi, chunki ular nafaqat antikorlarning barcha afzalliklariga ega, balki issiqlik barqarorligi, arzonligi va cheksiz qo'llanilishi kabi noyob afzalliklarga ega. Ushbu sharhda aptamerlarning antikorlarga alternativ sifatida tanilgan sabablarini keltiramiz. Bundan tashqari, in vitro tanlov jarayonlarining bir nechta turlari, jumladan nitroselüloza membranasini filtrlash, yaqinlik xromatografiyasi, magnit boncuk va kapillyar elektroforezga asoslangan tanlash usullari batafsil tushuntirilgan. Shuningdek, kasalliklar diagnostikasi va kichik molekulalarni aniqlash uchun aptamerlarning turli xil ilovalarini joriy qilamiz. Qulay modifikatsiyalari va aptamerlarning barqarorligi tufayli nishonlarni aniqlash uchun elektrokimyoviy, kolorimetrik, optik va massa sezgir usullar kabi ko'plab analitik usullardan foydalanish mumkin. Nihoyat, aptamerlarning bir nechta tibbiy va analitik ilovalari taqdim etiladi. Xulosa qilib aytganda, aptamerlar nafaqat antikorlarga muqobil, balki tibbiy va analitik uskunalarning asosiy komponentlari sifatida turli sohalar uchun istiqbolli materiallardir.
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References
Ishankhodjaev T. et al. Study on Effects of Liposomal Quercetin on Biochemical Parameters of the Nigrostriatal System of Rats with Experimentally Induced Neurodegenerative Disease //Annals of the Romanian Society for Cell Biology. – 2021. – С. 6128-6143.
Mukhammadjon M. et al. The effect of ngf on indicators of the antioxidant system in rat brain tissue //Universum: химия и биология. – 2021. – №. 9 (87). – С. 82-86.
Saatov T. et al. Antioxidant and hypoglycemic effects of gossitan //Endocrine Abstracts. – Bioscientifica, 2019. – Т. 63.
Saatov T. et al. Study on hypoglycemic effect of polyphenolic compounds isolated from the Euphorbia L. plants growing in uzbekistan //Endocrine Abstracts. – Bioscientifica, 2020. – Т. 70.
Saatov T. et al. Correction of oxidative stress in experimental diabetes mellitus by means of natural antioxidants //Endocrine Abstracts. – Bioscientifica, 2021. – Т. 73.
Irgasheva S. et al. Study on compositions of lipids in tissues of rats with alimentary obesity //Endocrine Abstracts. – Bioscientifica, 2019. – Т. 63.
Mamadalieva N. I., Mustafakulov M. A., Saatov T. S. The effect of nerve growth factor on indicators of the antioxidant system in rat brain tissue //eurasian union of scientists. series: medical, biological and chemical sciences Учредители: ООО" Логика+". – 2021. – №. 11. – С. 36-40.
Saatov T. et al. Study on antioxidant and hypoglycemic effects of natural polyphenols in the experimental diabetes model //Endocrine Abstracts. – Bioscientifica, 2018. – Т. 56.
Ishankhodjaev T. et al. Study on Effects of Liposomal Quercetin on Biochemical Parameters of the Nigrostriatal System of Rats with Experimentally Induced Neurodegenerative Disease //Annals of the Romanian Society for Cell Biology. – 2021. – С. 6128-6143.
Mukhammadjon M. et al. The effect of ngf on indicators of the antioxidant system in rat brain tissue //Universum: химия и биология. – 2021. – №. 9 (87). – С. 82-86.
Saatov T. et al. Antioxidant and hypoglycemic effects of gossitan //Endocrine Abstracts. – Bioscientifica, 2019. – Т. 63.
Saatov T. et al. Study on hypoglycemic effect of polyphenolic compounds isolated from the Euphorbia L. plants growing in uzbekistan //Endocrine Abstracts. – Bioscientifica, 2020. – Т. 70.
Saatov T. et al. Correction of oxidative stress in experimental diabetes mellitus by means of natural antioxidants //Endocrine Abstracts. – Bioscientifica, 2021. – Т. 73.
Irgasheva S. et al. Study on compositions of lipids in tissues of rats with alimentary obesity //Endocrine Abstracts. – Bioscientifica, 2019. – Т. 63.
Mamadalieva N. I., Mustafakulov M. A., Saatov T. S. The effect of nerve growth factor on indicators of the antioxidant system in rat brain tissue //eurasian union of scientists. series: medical, biological and chemical sciences Учредители: ООО" Логика+". – 2021. – №. 11. – С. 36-40.
Saatov T. et al. Study on antioxidant and hypoglycemic effects of natural polyphenols in the experimental diabetes model //Endocrine Abstracts. – Bioscientifica, 2018. – Т. 56.
Mustafakulov M. et al. Determination of antioxidant properties of l-cysteine in the liver of alloxan diabetes model rats //International Journal of Contemporary Scientific and Technical Research. – 2023. – №. Special Issue. – С. 47-54.
Мамадалиева Н. И., Мустафакулов М. А., Саатов Т. С. Влияние фактора нервного роста на показатели антиоксидантной системы в тканях мозга крысы //Environmental Science. – 2021. – Т. 723. – С. 022021.
Ishankhodjaev T. et al. Study on Effects of Liposomal Quercetin on Biochemical Parameters of the Nigrostriatal System of Rats with Experimentally Induced Neurodegenerative Disease //Annals of the Romanian Society for Cell Biology. – 2021. – С. 6128-6143.
Mukhammadjon M. et al. The effect of ngf on indicators of the antioxidant system in rat brain tissue //Universum: химия и биология. – 2021. – №. 9 (87). – С. 82-86.
Saatov T. et al. Antioxidant and hypoglycemic effects of gossitan //Endocrine Abstracts. – Bioscientifica, 2019. – Т. 63.
Saatov T. et al. Study on hypoglycemic effect of polyphenolic compounds isolated from the Euphorbia L. plants growing in uzbekistan //Endocrine Abstracts. – Bioscientifica, 2020. – Т. 70.
Saatov T. et al. Correction of oxidative stress in experimental diabetes mellitus by means of natural antioxidants //Endocrine Abstracts. – Bioscientifica, 2021. – Т. 73.
Irgasheva S. et al. Study on compositions of lipids in tissues of rats with alimentary obesity //Endocrine Abstracts. – Bioscientifica, 2019. – Т. 63.
Mamadalieva N. I., Mustafakulov M. A., Saatov T. S. The effect of nerve growth factor on indicators of the antioxidant system in rat brain tissue //eurasian union of scientists. series: medical, biological and chemical sciences Учредители: ООО" Логика+". – 2021. – №. 11. – С. 36-40.
Saatov T. et al. Study on antioxidant and hypoglycemic effects of natural polyphenols in the experimental diabetes model //Endocrine Abstracts. – Bioscientifica, 2018. – Т. 56.
Mustafakulov M. et al. Determination of antioxidant properties of l-cysteine in the liver of alloxan diabetes model rats //International Journal of Contemporary Scientific and Technical Research. – 2023. – №. Special Issue. – С. 47-54.
Мамадалиева Н. И., Мустафакулов М. А., Саатов Т. С. Влияние фактора нервного роста на показатели антиоксидантной системы в тканях мозга крысы //Environmental Science. – 2021. – Т. 723. – С. 022021.
Saatov T. et al. Correction of oxidative stress in experimental diabetes mellitus by means of natural antioxidants //Endocrine Abstracts. – Bioscientifica, 2021. – Т. 73.
Saatov T. et al. Study on hypoglycemic effect of polyphenolic compounds isolated from the Euphorbia L. plants growing in uzbekistan //Endocrine Abstracts. – Bioscientifica, 2020. – Т. 70.
Saatov T. et al. Antioxidant and hypoglycemic effects of gossitan //Endocrine Abstracts. – Bioscientifica, 2019. – Т. 63.
Ishankhodjaev T. et al. Study on Effects of Liposomal Quercetin on Biochemical Parameters of the Nigrostriatal System of Rats with Experimentally Induced Neurodegenerative Disease //Annals of the Romanian Society for Cell Biology. – 2021. – С. 6128-6143.
Saatov T. et al. Neurodegeneration type and severity have linkage with plasma insulin in DM patients //Endocrine Abstracts. – Bioscientifica, 2022. – Т. 81.
Irgasheva S. va boshqalar. TNF-a gen G308A polimorfizmi: 2-toifa qandli diabet bilan og'rigan bemorlarda chastota // Int J Cur Res Rev. – 2020. – T. 2020. – B. 161.
Mustafakulov M. et al. Determination of antioxidant properties of l-cysteine in the liver of alloxan diabetes model rats //International Journal of Contemporary Scientific and Technical Research. – 2023. – №. Special Issue. – С. 47-54.
Shamansurova Z. et al. Plasma Insulin level have linkage with neurodegeneration in patients with type 2 DM //Physiology. – 2023. – Т. 38. – №. S1. – С. 5732129.
Mallayeva M., Mustafakulov M. Toksik gepatitda lipidlarning peroksidalnish mahsulotlariga polifenollarning ta’siri //International Journal of scientific and Applied Research. – 2024. – Т. 1. – №. 3. – С. 75-78.
Uralov A. et al. Building the inflower and realizing seed productivity of A. giganteum regel //E3S Web of Conferences. – EDP Sciences, 2024. – Т. 538. – С. 03020.
Mustafoqulov M. va boshqalar. Mikroorganizmlardan insulinni genetik muhendislik texnologiyasi //Zamonaviy ilmiy va texnik tadqiqotlar xalqaro jurnali. – 2023. – T. 1. – Yoʻq. 2. – 4-12-betlar.
Mustafoqulov M., Mamadaliyeva Z. TRANSGEN HAYVON OLISH USULLARI //International Journal of Science and Applied Research. – 2024. – T. 1. – Yoʻq. 3. – 78-81-betlar.
